REMINDER: Two Sample Rule
To ensure patient safety, the transfusion laboratory requires the patient to have been tested on more than one occasion prior to issuing red blood cells. The two separate samples for Group and Save must have been taken at different times, i.e at different phlebotomy sessions.
It is not acceptable to collect two blood samples at the same time and label them a few minutes apart. Such specimens will be rejected.
REMINDER: Labelling Blood Transfusion Specimens and Request Forms
All specimen tubes MUST be labelled with 3 key identifiers:
- Patient’s last name
- Patient’s first name
- NHS number or Trakcare registration number
Blood tubes must be signed and dated.
Addressograph labels, unclear or incorrectly labelled samples will be REJECTED in accordance with national guidance.
Transfusion Investigations Available
|Investigation||Specimen Type & Volume||Average Turnaround Time||Comments|
|Cross Match||1 x pink top EDTA (6ml)||Depends on urgency||Can be performed on G&S sample|
|Group & Save (G&S)||1 x pink top EDTA (6ml)||6 hours (Complex investigations that are sent away = 7 days)||Refer to ‘Two Sample Rule’|
|Antenatal Screening||1 x pink top EDTA (6ml)||4-5 hours (Complex investigations that are sent away = 7 days)|
|Foetal Leak (Includes Kleihauer where indicated)||1 x pink top EDTA (6ml) – Maternal 1 x purple top EDTA (3.5ml) – Cord||4 hours|
|Baby Group & Direct Antiglobulin Test (DAT)||1 x purple top EDTA (1ml, paediatric)||2 hours|
|Baby Cross Match||1 x pink top EDTA (6ml) – Maternal1 x purple top EDTA (1ml Paediatric) – Baby||Depends on urgency||Neonatal cross match is performed against the maternal sample up to the age of 3 months.|
|Kleihauer Test||1 x purple top EDTA (3.5ml)||4 hours||Test can be performed on a current FBC sample.|
|Direct Antiglobulin Test||1 x purple top EDTA (3.5ml)||5 – 6 hours||Test can be performed on a current FBC sample.|
|HIT||1 x pink top EDTA (6ml)1 x gold top serum (3.5ml)||7 days|
|HLA Typing||1 x pink top EDTA (6ml)||7 days|
|HLA Antibody Screen||1 x gold top serum (3.5ml)||7 days|
|HLA Typing for disease association||1 x pink top EDTA (6ml)||7 days|
For investigations not listed above, please contact the Transfusion laboratory on 01271 322327 (or internal ext. 2327)
NOTE: If printed, information on this page is only valid on day of printing.