Blood Transfusion Investigations

REMINDER: Two Sample Rule

To ensure patient safety, the transfusion laboratory requires the patient to have been tested on more than one occasion prior to issuing red blood cells.  The two separate samples for Group and Save must have been taken at different times, i.e at different phlebotomy sessions.

It is not acceptable to collect two blood samples at the same time and label them a few minutes apart. Such specimens will be rejected.

REMINDER: Labelling Blood Transfusion Specimens and Request Forms

All specimen tubes MUST be labelled with 3 key identifiers:

  • Patient’s last name
  • Patient’s first name
  • DOB
  • NHS number or Trakcare registration number

Blood tubes must be signed and dated.

Addressograph labels, unclear or incorrectly labelled samples will be REJECTED in accordance with national guidance.

Transfusion Investigations Available

InvestigationSpecimen Type & Volume Average Turnaround TimeComments
Cross Match1 x pink top EDTA (6ml)Depends on urgencyCan be performed on G&S sample
Group & Save (G&S)1 x pink top EDTA (6ml)6 hours (Complex investigations that are sent away = 7 days)Refer to ‘Two Sample Rule’
Antenatal Screening1 x pink top EDTA (6ml)4-5 hours (Complex investigations that are sent away = 7 days)
Foetal Leak (Includes Kleihauer where indicated)1 x pink top EDTA (6ml) – Maternal 1 x purple top EDTA (3.5ml) – Cord4 hours
Baby Group & Direct Antiglobulin Test (DAT)1 x purple top EDTA (1ml, paediatric)2 hours
Baby Cross Match1 x pink top EDTA (6ml) – Maternal1 x purple top EDTA (1ml Paediatric) – BabyDepends on urgencyNeonatal cross match is performed against the maternal sample up to the age of 3 months.
Kleihauer Test1 x purple top EDTA (3.5ml)4 hoursTest can be performed on a current FBC sample.
Direct Antiglobulin Test1 x purple top EDTA (3.5ml)5 – 6 hoursTest can be performed on a current FBC sample.
HIT1 x pink top EDTA (6ml)1 x gold top serum (3.5ml)7 days
HLA Typing1 x pink top EDTA (6ml)7 days
HLA Antibody Screen1 x gold top serum (3.5ml)7 days
HLA Typing for disease association1 x pink top EDTA (6ml)7 days

For investigations not listed above, please contact the Transfusion laboratory on 01271 322327 (or internal ext. 2327)

NOTE: If printed, information on this page is only valid on day of printing.

Last updated: May 10, 2018